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PR2026-ST01
Bioteke
Multiplex Fluorescent PCR for Accurate&Efficient Pathogen Detection
(FOR PROFESSIONAL USE ONLY)
The kit is used for qualitative screening test of 14 common STIs pathogens nucleic acid in vaginal swab and urine specimens from sexually transmitted infection suspected patients.
This kit is a multiplex fluorecent probe-based Taqman® qPCR assay system. Specific primers and probes are designed for the detection of specific genes of 14 common STI/STD pathogens.
Internal control(IC) serves as the monitor to confirm successful extraction and identify possible PCR inhibition for the entire testing system to prevent false negative detection results.In order to avoid aerosol contamination of the amplified products, the UDG enzyme /dUTP system was added to the amplification system to effectively degrade the amplified products and avoid false positive results.
This kit is a fully premix freeze-dried system. Taq enzyme, UDG enzyme, reaction buffer, specific primers and probes required for amplification are all lyophilized in PCR tubes. Detection can be performed directly after adding dissolving solution and extracted nucleic acid.
Targets | Trichomonas vaginalis |
Targets | Ureaplasma urealyticum |
| Neisseria gonorrhoeae | Ureaplasma parvum | ||
| Chlamydia trachomatis | Mycoplasma hominis | ||
| Gardnerella vaginalis | Mycoplasma genitalium | ||
| Haemophilus ducreyi | Treponema pallidum | ||
| Candida albicans | Herpes simplex virus type 1 | ||
| Streptococcus agalactiae | Herpes simplex virus type 2 |
| Components | 8 samples/kit | 24 samples/kit | Ingredient |
| ST01 Lyophilized Reagent | 8*4 strip tubes | 24*4 strip tubes | Specific primer&probes, dNTP/dUTP, Mg2+, Taq polymerase and UDG enzyme |
| STI Lysis Buffer | 1 mL*1 tube | 5 mL*1 tube | Surface active agent, balanced salt solution |
| One Test Glass Beads | 8 tubes*1 bag | 8 tubes*3 bags | Glass beads |
| STI Positive Control | 400 μL*1 tube | 400 μL*1 tube | Plasmid containing every target gene sequence |
| STI Negative Control | 400 μL*1 tube | 400 μL*1 tube | Plasmid containing internal control sequence |
| Dissolving Solution | 1 mL*1 tube | 1 mL*2 tubes | Stabilizer |
Store frozen at -20±5℃.
The kit can be transported at room temperature (≤ 1 month).
Repeated freezing and thawing should not exceed 7 times.
Valid for 12 months(From the QC passed).
Disposable powder free gloves and other personal protective equipment.
Pipettes (adjustable) and Sterile pipette tips.
Vaginal swabs/urine sample collection device.
1.5 mL centrifuge tubes and racks.
Bench top centrifuge for centrifuge tubes and PCR tubes.
Vortex mixer.(Power≥40w)
Centrifuge tube holder for vortex mixer. (not necessary,can replace part of manual operation)
Metal bath/water bath.(1.5mL centrifuge tube,95℃)
Real-time PCR instrument with FAM/VIC/ROX/CY5 fluorescence channels.(Such as ABI7500, Bio-rad CFX96, QuantStudio, SLAN-96S, BTK-96, BTK-16)
Detection limitation: 1000 Copies/mL.
Precision: The coefficient of variation (CV, %) of Ct value of within-batch/between-batch precision is ≤ 5%.
Accuracy: The conformity rate of negative/positive reference: 100%.
Specificity:The target organisms had been analyzed in silico for potential cross-reactivity with the primers or probe sequences.Homology with other species is all below 90%. Specificity analysis showed that there is no cross reaction between the pathogens detected by this kit.
▇ Bioteke uses a fully automated nucleic acid extraction and PCR testing device, coupled with a patented STI screening kit, to achieve a one-step diagnosis of “sample in, result out”
▇ Features
1. Fast & Simple
No need to extract nucleic acid, the sample can be directly put into the machine after adding lysis buffer. The whole process only takes 75-90 minutes.
2. Accurate & Efficient
Reduce manual operation errors and shorten detection time.
3. Reduce Cost
Reduce experimental consumables and labor costs.
4. Flexible & Convenient
Can be used for small batch testing and instant testing(POCT).
5. Security & Compliance
The product has IVD CE / MSDS / ISO 13485 certificates.
Click here to learn more
※ Fully Automated Sexually Transmitted Infection(STI) Screening Solution
※ Watch the video of operation process
▲ Human Papillomavirus HPV Genotyping Detection Kit (Fluorescence PCR Method)
▲ Urinary Tract Infection (UTI) & DR Panel (Fluorescence PCR Method)
▲ Multiple Respiratory Pathogens Nucleic Acid Detection Kit (Fluorescence PCR Method)
▲ Wound Pathogen Panel(Fluorescence PCR Method)
Multiplex Fluorescent PCR for Accurate&Efficient Pathogen Detection
(FOR PROFESSIONAL USE ONLY)
The kit is used for qualitative screening test of 14 common STIs pathogens nucleic acid in vaginal swab and urine specimens from sexually transmitted infection suspected patients.
This kit is a multiplex fluorecent probe-based Taqman® qPCR assay system. Specific primers and probes are designed for the detection of specific genes of 14 common STI/STD pathogens.
Internal control(IC) serves as the monitor to confirm successful extraction and identify possible PCR inhibition for the entire testing system to prevent false negative detection results.In order to avoid aerosol contamination of the amplified products, the UDG enzyme /dUTP system was added to the amplification system to effectively degrade the amplified products and avoid false positive results.
This kit is a fully premix freeze-dried system. Taq enzyme, UDG enzyme, reaction buffer, specific primers and probes required for amplification are all lyophilized in PCR tubes. Detection can be performed directly after adding dissolving solution and extracted nucleic acid.
Targets | Trichomonas vaginalis |
Targets | Ureaplasma urealyticum |
| Neisseria gonorrhoeae | Ureaplasma parvum | ||
| Chlamydia trachomatis | Mycoplasma hominis | ||
| Gardnerella vaginalis | Mycoplasma genitalium | ||
| Haemophilus ducreyi | Treponema pallidum | ||
| Candida albicans | Herpes simplex virus type 1 | ||
| Streptococcus agalactiae | Herpes simplex virus type 2 |
| Components | 8 samples/kit | 24 samples/kit | Ingredient |
| ST01 Lyophilized Reagent | 8*4 strip tubes | 24*4 strip tubes | Specific primer&probes, dNTP/dUTP, Mg2+, Taq polymerase and UDG enzyme |
| STI Lysis Buffer | 1 mL*1 tube | 5 mL*1 tube | Surface active agent, balanced salt solution |
| One Test Glass Beads | 8 tubes*1 bag | 8 tubes*3 bags | Glass beads |
| STI Positive Control | 400 μL*1 tube | 400 μL*1 tube | Plasmid containing every target gene sequence |
| STI Negative Control | 400 μL*1 tube | 400 μL*1 tube | Plasmid containing internal control sequence |
| Dissolving Solution | 1 mL*1 tube | 1 mL*2 tubes | Stabilizer |
Store frozen at -20±5℃.
The kit can be transported at room temperature (≤ 1 month).
Repeated freezing and thawing should not exceed 7 times.
Valid for 12 months(From the QC passed).
Disposable powder free gloves and other personal protective equipment.
Pipettes (adjustable) and Sterile pipette tips.
Vaginal swabs/urine sample collection device.
1.5 mL centrifuge tubes and racks.
Bench top centrifuge for centrifuge tubes and PCR tubes.
Vortex mixer.(Power≥40w)
Centrifuge tube holder for vortex mixer. (not necessary,can replace part of manual operation)
Metal bath/water bath.(1.5mL centrifuge tube,95℃)
Real-time PCR instrument with FAM/VIC/ROX/CY5 fluorescence channels.(Such as ABI7500, Bio-rad CFX96, QuantStudio, SLAN-96S, BTK-96, BTK-16)
Detection limitation: 1000 Copies/mL.
Precision: The coefficient of variation (CV, %) of Ct value of within-batch/between-batch precision is ≤ 5%.
Accuracy: The conformity rate of negative/positive reference: 100%.
Specificity:The target organisms had been analyzed in silico for potential cross-reactivity with the primers or probe sequences.Homology with other species is all below 90%. Specificity analysis showed that there is no cross reaction between the pathogens detected by this kit.
▇ Bioteke uses a fully automated nucleic acid extraction and PCR testing device, coupled with a patented STI screening kit, to achieve a one-step diagnosis of “sample in, result out”
▇ Features
1. Fast & Simple
No need to extract nucleic acid, the sample can be directly put into the machine after adding lysis buffer. The whole process only takes 75-90 minutes.
2. Accurate & Efficient
Reduce manual operation errors and shorten detection time.
3. Reduce Cost
Reduce experimental consumables and labor costs.
4. Flexible & Convenient
Can be used for small batch testing and instant testing(POCT).
5. Security & Compliance
The product has IVD CE / MSDS / ISO 13485 certificates.
Click here to learn more
※ Fully Automated Sexually Transmitted Infection(STI) Screening Solution
※ Watch the video of operation process
▲ Human Papillomavirus HPV Genotyping Detection Kit (Fluorescence PCR Method)
▲ Urinary Tract Infection (UTI) & DR Panel (Fluorescence PCR Method)
▲ Multiple Respiratory Pathogens Nucleic Acid Detection Kit (Fluorescence PCR Method)
▲ Wound Pathogen Panel(Fluorescence PCR Method)
Telephone: +86-510-8332 3992
