Human Papillomavirus(HPV) Genotyping Detection Kit

For Research Use Only

• The high-risk HPV includes type 16,18,26,31,33,35,39,45,51,52,53,56,58,59,66,68,73,82; 

• The low-risk HPV includes type 6,11,40,42,43,44/55,54,57/71,61,70,72,81,84.

  
  

Note:

Supports customized detection target combinations(OEM)

• Specific primers and probes are designed for detection of different types of human papillomavirus (HPV).

• Internal control (IC) is serves as the monitor to confirm successful extraction & identify possible PCR inhibition for the entire testing system to prevent false negative detection results.

• UDG enzyme/dUTP system was added to the amplification system to effectively degrade the amplified products & avoid false positive results.

• This kit is a fully premixed lyophilized system. Taq enzyme, UDG enzyme, reaction buffer, specific primers and probes required for amplification are all lyophilized in PCR tubes.

Conponents	8 samples/kit	24 samples/kit	Ingredient
HP04 Lyophilized Reagent	8×8 strip tubes	24×8 strip tubes	Specific primer&probes for the detection of target pathogens, dNTP/dUTP Mix, Mg2+, Tac polymerase and UDG enzyme
HPV Lysis Buffer	1 mL×1 tube	2.5 mL×1 tube	Surface active agent, balanced salt solution
HPV Positive Control	400 mL×1 tube	400 mL×1 tube	Plasmid containing every target gene sequence
HPV Negative Control	400 mL×1 tube	400 mL×1 tube	Plasmid containing internal control sequence
Dissolving Solution	1 mL×1 tube	1 mL×3 tubes	Stabilizer

NOTE:

1. The reagents repeated freezing and thawing should not exceed 7 times.

2. The kit can be transported at room temperature. (No more than 1 month)

PCR Amplification

1. Put the reaction tubes into PCR instrument and set the names of each reaction well in the corresponding order.

2. Select FAM,VIC/HEX,ROX and CY5 four fluorescence channels for detection.The corresponding targets of each fluorescence channel are shown in the table below:

   
   

Location	FAM	VIC/HEX	ROX	CY5
Well 1	HPV type 16	HPV type 18	HPV type 26	Internal Control (IC)
Well 2	HPV type 31	HPV type 33	HPV type 35	HPV type 39
Well 3	HPV type 45	HPV type 51	HPV type 52	HPV type 53
Well 4	HPV type 56	HPV type 58	HPV type 59	HPV type 68
Well 5	HPV type 66	HPV type 73	HPV type 82	HPV type 81
Well 6	HPV type 6	HPV type 11	HPV type 40	HPV type 42
Well 7	HPV type 43	HPV type 44/55	HPV type 54	HPV type 61
Well 8	HPV type 57/71	HPV type 70	HPV type 72	HPV type 84

2. Precision: The coefficient of variation (CV,%) of Ct value of within-batch/between-batch precision is≤5%.

3. Accuracy: The conformity rate of negative/positive reference:100%.

4. Specificity:The farget organisms had been analyzed in silico for potential cross-reactivity with the primers or probe sequences.

Bioteke HP04 Brochure.pdf