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PR2026-HP04
Bioteke
For Research Use Only
The high-risk HPV includes type 16,18,26,31,33,35,39,45,51,52,53,56,58,59,66,68,73,82;
The low-risk HPV includes type 6,11,40,42,43,44/55,54,57/71,61,70,72,81,84.
Specific primers and probes are designed for detection of different types of human papillomavirus (HPV).
Internal control (IC) is serves as the monitor to confirm successful extraction & identify possible PCR inhibition for the entire testing system to prevent false negative detection results.
UDG enzyme/dUTP system was added to the amplification system to effectively degrade the amplified products & avoid false positive results.
This kit is a fully premixed lyophilized system. Taq enzyme, UDG enzyme, reaction buffer, specific primers and probes required for amplification are all lyophilized in PCR tubes.
Model: PR2026-HP04
Format: Test cassette
Storage: -25~-15℃(-13~5℉)
Shelf life: 12 months
| Conponents | 8 samples/kit | 24 samples/kit | Ingredient |
| HP04 Lyophilized Reagent | 8×8 strip tubes | 24×8 strip tubes | Specific primer&probes for the detection of target pathogens, dNTP/dUTP Mix, Mg2+, Tac polymerase and UDG enzyme |
| HPV Lysis Buffer | 1 mL×1 tube | 2.5 mL×1 tube | Surface active agent, balanced salt solution |
| HPV Positive Control | 400 mL×1 tube | 400 mL×1 tube | Plasmid containing every target gene sequence |
| HPV Negative Control | 400 mL×1 tube | 400 mL×1 tube | Plasmid containing internal control sequence |
| Dissolving Solution | 1 mL×1 tube | 1 mL×3 tubes | Stabilizer |
NOTE:
1. The reagents repeated freezing and thawing should not exceed 7 times.
2. The kit can be transported at room temperature. (No more than 1 month)
PCR Amplification
Put the reaction tubes into PCR instrument and set the names of each reaction well in the corresponding order.
Select FAM,VIC/HEX,ROX and CY5 four fluorescence channels for detection.The corresponding targets of each fluorescence channel are shown in the table below:
| Location | FAM | VIC/HEX | ROX | CY5 |
| Well 1 | HPV type 16 | HPV type 18 | HPV type 26 | Internal Control (IC) |
| Well 2 | HPV type 31 | HPV type 33 | HPV type 35 | HPV type 39 |
| Well 3 | HPV type 45 | HPV type 51 | HPV type 52 | HPV type 53 |
| Well 4 | HPV type 56 | HPV type 58 | HPV type 59 | HPV type 68 |
| Well 5 | HPV type 66 | HPV type 73 | HPV type 82 | HPV type 81 |
| Well 6 | HPV type 6 | HPV type 11 | HPV type 40 | HPV type 42 |
| Well 7 | HPV type 43 | HPV type 44/55 | HPV type 54 | HPV type 61 |
| Well 8 | HPV type 57/71 | HPV type 70 | HPV type 72 | HPV type 84 |
2. Precision: The coefficient of variation (CV,%) of Ct value of within-batch/between-batch precision is≤5%.
3. Accuracy: The conformity rate of negative/positive reference:100%.
4. Specificity:The farget organisms had been analyzed in silico for potential cross-reactivity with the primers or probe sequences.
For Research Use Only
The high-risk HPV includes type 16,18,26,31,33,35,39,45,51,52,53,56,58,59,66,68,73,82;
The low-risk HPV includes type 6,11,40,42,43,44/55,54,57/71,61,70,72,81,84.
Specific primers and probes are designed for detection of different types of human papillomavirus (HPV).
Internal control (IC) is serves as the monitor to confirm successful extraction & identify possible PCR inhibition for the entire testing system to prevent false negative detection results.
UDG enzyme/dUTP system was added to the amplification system to effectively degrade the amplified products & avoid false positive results.
This kit is a fully premixed lyophilized system. Taq enzyme, UDG enzyme, reaction buffer, specific primers and probes required for amplification are all lyophilized in PCR tubes.
Model: PR2026-HP04
Format: Test cassette
Storage: -25~-15℃(-13~5℉)
Shelf life: 12 months
| Conponents | 8 samples/kit | 24 samples/kit | Ingredient |
| HP04 Lyophilized Reagent | 8×8 strip tubes | 24×8 strip tubes | Specific primer&probes for the detection of target pathogens, dNTP/dUTP Mix, Mg2+, Tac polymerase and UDG enzyme |
| HPV Lysis Buffer | 1 mL×1 tube | 2.5 mL×1 tube | Surface active agent, balanced salt solution |
| HPV Positive Control | 400 mL×1 tube | 400 mL×1 tube | Plasmid containing every target gene sequence |
| HPV Negative Control | 400 mL×1 tube | 400 mL×1 tube | Plasmid containing internal control sequence |
| Dissolving Solution | 1 mL×1 tube | 1 mL×3 tubes | Stabilizer |
NOTE:
1. The reagents repeated freezing and thawing should not exceed 7 times.
2. The kit can be transported at room temperature. (No more than 1 month)
PCR Amplification
Put the reaction tubes into PCR instrument and set the names of each reaction well in the corresponding order.
Select FAM,VIC/HEX,ROX and CY5 four fluorescence channels for detection.The corresponding targets of each fluorescence channel are shown in the table below:
| Location | FAM | VIC/HEX | ROX | CY5 |
| Well 1 | HPV type 16 | HPV type 18 | HPV type 26 | Internal Control (IC) |
| Well 2 | HPV type 31 | HPV type 33 | HPV type 35 | HPV type 39 |
| Well 3 | HPV type 45 | HPV type 51 | HPV type 52 | HPV type 53 |
| Well 4 | HPV type 56 | HPV type 58 | HPV type 59 | HPV type 68 |
| Well 5 | HPV type 66 | HPV type 73 | HPV type 82 | HPV type 81 |
| Well 6 | HPV type 6 | HPV type 11 | HPV type 40 | HPV type 42 |
| Well 7 | HPV type 43 | HPV type 44/55 | HPV type 54 | HPV type 61 |
| Well 8 | HPV type 57/71 | HPV type 70 | HPV type 72 | HPV type 84 |
2. Precision: The coefficient of variation (CV,%) of Ct value of within-batch/between-batch precision is≤5%.
3. Accuracy: The conformity rate of negative/positive reference:100%.
4. Specificity:The farget organisms had been analyzed in silico for potential cross-reactivity with the primers or probe sequences.
