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PR2024-UT01
BioTeke
Intended Use
Urinary Tract Infection (UTI) & Drug Resistance (DR) Gene Detection Kit is qualitative in vitro test of 19 common pathogens DNA and 12 common drug resistance (DR) genes in urine specimens obtained from individuals with urinary tract infections (UTI).
The pathogens and drug resistance genes detected are shown in the following [table 1].
[table 1]
| Pathogen & DR gene type | Species |
Bacteria | Escherichia coli |
| Klebsiella aerogenes | |
| Klebsiella oxytoca | |
| Klebsiella pneumoniae | |
| Citrobacter spp.1 | |
| Enterobacter cloacae | |
| Acinetobacter baumannii | |
| Proteus mirabilis | |
| Pseudomonas aeruginosa | |
| Serratia marcescens | |
| Staphylococcus aureus | |
| Staphylococcus saprophyticus | |
| Streptococcus agalactiae | |
| Enterococcus faecalis | |
| Enterococcus faecium | |
| Morganella morganii | |
| Bacteroides fragilis | |
| Staphylococcus epidermidis | |
| Fungi | Candida albicans |
Drug resistance gene | blaKPC |
| blaNDM | |
| blaVIM | |
| blaIMP | |
| blaOXA-48 | |
| mecA | |
| vanA | |
| vanB | |
| CTX-M2 | |
| suL1 | |
| suL2 | |
| suL3 |
1. Include：Citrobacter freundii, Citrobacter werkmanii, Citrobacter portucalensis, Citrobacter youngae, Citrobacter cronae, Citrobacter braakii, Citrobacter arsenatis, Citrobacter amalonaticus, Citrobacter koseri.
2. Include：CTX-M 1 group, CTX-M 2 group, CTX-M 9 group.
Principle
Urinary Tract Infection (UTI) & Drug Resistance (DR) Gene Detection Kit is designed with specific primers and probes for different common pathogens and drug resistance genes. Polymerase chain reaction (qPCR) and multiple fluorescent probe technique are used to amplify and detect specific nucleic acid sequences of pathogens and drug resistance genes listed in Table 1.
By adding edible yeast as internal control (IC), the extraction and detection process of pathogen nucleic acid in urine is monitored to effectively avoid false negative results. In order to avoid aerosol contamination of the amplified products, the UDG enzyme /dUTP system was added to the amplification system to effectively degrade the amplified products and avoid false positive results.
This kit is a fully premix freeze-dried system. Taq enzyme, UDG enzyme, reaction buffer, specific primers and probes required for amplification are all lyophilized in PCR tubes. A total of 1-8 Wells are lyophilized powders for different target genes. Detection can be performed directly after adding dissolving solution and extracted nucleic acid.
Operation Process
Advantages
Storage Conditions and Shelf Life
Store at -20℃±5℃
Store at 4℃ for no more than 6 months.
Transport at room temperature for no more than 1 month.
Valid for 12 months
Components
UTI lyophilized reagents
Lysis buffer
IC Dry powder & IC solution
One test glass beads
UTI Positive control / UTI Negative control
UTI Dissolving solution
Specimens Requirements
1). Urine samples, collected samples should be used for detection within the same day.
2). Stored at 2-8℃ less than 7 days. Stored at ＜20℃ for 6 months.
Performance
1). Detection limitation
200 CFU/mL
2). Precision: (CV, %) of Ct ≤5%
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Wound infection pcr panel
HPV genotyping kit
Intended Use
Urinary Tract Infection (UTI) & Drug Resistance (DR) Gene Detection Kit is qualitative in vitro test of 19 common pathogens DNA and 12 common drug resistance (DR) genes in urine specimens obtained from individuals with urinary tract infections (UTI).
The pathogens and drug resistance genes detected are shown in the following [table 1].
[table 1]
| Pathogen & DR gene type | Species |
Bacteria | Escherichia coli |
| Klebsiella aerogenes | |
| Klebsiella oxytoca | |
| Klebsiella pneumoniae | |
| Citrobacter spp.1 | |
| Enterobacter cloacae | |
| Acinetobacter baumannii | |
| Proteus mirabilis | |
| Pseudomonas aeruginosa | |
| Serratia marcescens | |
| Staphylococcus aureus | |
| Staphylococcus saprophyticus | |
| Streptococcus agalactiae | |
| Enterococcus faecalis | |
| Enterococcus faecium | |
| Morganella morganii | |
| Bacteroides fragilis | |
| Staphylococcus epidermidis | |
| Fungi | Candida albicans |
Drug resistance gene | blaKPC |
| blaNDM | |
| blaVIM | |
| blaIMP | |
| blaOXA-48 | |
| mecA | |
| vanA | |
| vanB | |
| CTX-M2 | |
| suL1 | |
| suL2 | |
| suL3 |
1. Include：Citrobacter freundii, Citrobacter werkmanii, Citrobacter portucalensis, Citrobacter youngae, Citrobacter cronae, Citrobacter braakii, Citrobacter arsenatis, Citrobacter amalonaticus, Citrobacter koseri.
2. Include：CTX-M 1 group, CTX-M 2 group, CTX-M 9 group.
Principle
Urinary Tract Infection (UTI) & Drug Resistance (DR) Gene Detection Kit is designed with specific primers and probes for different common pathogens and drug resistance genes. Polymerase chain reaction (qPCR) and multiple fluorescent probe technique are used to amplify and detect specific nucleic acid sequences of pathogens and drug resistance genes listed in Table 1.
By adding edible yeast as internal control (IC), the extraction and detection process of pathogen nucleic acid in urine is monitored to effectively avoid false negative results. In order to avoid aerosol contamination of the amplified products, the UDG enzyme /dUTP system was added to the amplification system to effectively degrade the amplified products and avoid false positive results.
This kit is a fully premix freeze-dried system. Taq enzyme, UDG enzyme, reaction buffer, specific primers and probes required for amplification are all lyophilized in PCR tubes. A total of 1-8 Wells are lyophilized powders for different target genes. Detection can be performed directly after adding dissolving solution and extracted nucleic acid.
Operation Process
Advantages
Storage Conditions and Shelf Life
Store at -20℃±5℃
Store at 4℃ for no more than 6 months.
Transport at room temperature for no more than 1 month.
Valid for 12 months
Components
UTI lyophilized reagents
Lysis buffer
IC Dry powder & IC solution
One test glass beads
UTI Positive control / UTI Negative control
UTI Dissolving solution
Specimens Requirements
1). Urine samples, collected samples should be used for detection within the same day.
2). Stored at 2-8℃ less than 7 days. Stored at ＜20℃ for 6 months.
Performance
1). Detection limitation
200 CFU/mL
2). Precision: (CV, %) of Ct ≤5%
[You May Like]
Wound infection pcr panel
HPV genotyping kit
