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PR2028-WP01
BioTeke
The Wound Pathogen Panel is qualitative in vitro detection of 19 common pathogens DNA and 12 common drug(antibiotics) resistance(DR) genes from wound swab.
The pathogens and drug resistance genes detected are shown in the following [table 1].
[Table 1]
| Pathogen & DR gene type | Species |
Bacteria | Escherichia coli |
| Klebsiella aerogenes | |
| Klebsiella oxytoca | |
| Klebsiella pneumoniae | |
| Citrobacter spp.1 | |
| Enterobacter cloacae | |
| Acinetobacter baumannii | |
| Proteus mirabilis | |
| Pseudomonas aeruginosa | |
| Serratia marcescens | |
| Staphylococcus aureus | |
| Staphylococcus saprophyticus | |
| Streptococcus agalactiae | |
| Enterococcus faecalis | |
| Enterococcus faecium | |
| Morganella morganii | |
| Bacteroides fragilis | |
| Staphylococcus epidermidis | |
| Fungi | Candida albicans |
Drug resistance gene | blaKPC |
| blaNDM | |
| blaVIM | |
| blaIMP | |
| blaOXA-48 | |
| mecA | |
| vanA | |
| vanB | |
| CTX-M2 | |
| suL1 | |
| suL2 | |
| suL3 |
1. Include：Citrobacter freundii, Citrobacter werkmanii, Citrobacter portucalensis, Citrobacter youngae, Citrobacter cronae, Citrobacter braakii, Citrobacter arsenatis, Citrobacter amalonaticus, Citrobacter koseri.
2. Include：CTX-M 1 group, CTX-M 2 group, CTX-M 9 group.
The kit is designed with specific primers and probes for different common pathogens and drug resistance genes. Polymerase chain reaction (qPCR) and multiple fluorescent probe technique are used to amplify and detect specific nucleic acid sequences of pathogens and drug resistance genes listed in Table 1.
By adding edible yeast as internal control (IC), the extraction and detection process of pathogen nucleic acid from wound swab is monitored to effectively avoid false negative results. In order to avoid aerosol contamination of the amplified products, the UDG enzyme /dUTP system was added to the amplification system to effectively degrade the amplified products and avoid false positive results.
This kit is a fully premix freeze-dried system. Taq enzyme, UDG enzyme, reaction buffer, specific primers and probes required for amplification are all lyophilized in PCR tubes. A total of 1-8 Wells are lyophilized powders for different target genes. Detection can be performed directly after adding dissolving solution and extracted nucleic acid.
Model: PR2028-WP01
Format: Test cassette
Specimen: Wound swab
Storage: -25~-15℃(-13~5℉)
Shelf life: 12 months
| Components | 8 samples/kit | 24 samples/kit | Ingredient |
Wound Lyophilized Reagent | 8×8 strip tubes | 24×8 strip tubes | Specific primer&probes for the detection of pathogens and DR genes in Table 1, dNTP/dUTP Mix, Mg2+, Taq polymerase and UDG enzyme |
Internal Control Dry Powder | 1 tube | 1 tube | Edible yeast powder |
Internal Control Solution | 1 mL×1 tube | 1 mL×1 tube | Dnase/Rnase-free H₂O |
| Wound Positive Control | 300 μL×1 tube | 300 μL×1 tube | Plasmid containing every target gene sequence |
| Wound Negative Control | 300 μL×1 tube | 300 μL×1 tube | Plasmid containing internal control sequence |
| Dissolving Solution | 1 mL×1 tube | 1 mL×3 tubes | Stabilizer |
NOTE:
1. Do not mix the components from different batches for detection.
2. The kit can be transported at room temperature (no more than one month).
3. Repeated freezing and thawing should not exceed 7 times.
2). Precision: (CV, %) of Ct ≤5%.
3). Accuracy: The conformity rate of negative/positive reference 100%.
4). Specificity: Please refer to the User Manual.
1. Disposable powder free gloves and other personal protective equipment；
2. Pipettes (adjustable) and Sterile pipette tips；
3. Wound swab collection device；
4. 1.5 mL centrifuge tubes and racks；
5. Bench top centrifuge for centrifuge tubes and PCR tubes；
6. Bacterial nucleic acid extraction kit(It is recommended to use Nucleic Acid Extration Reagent manufactured by Bioteke, Item No.: BUR01)；
7. Nucleic acid extractor；
8. Metal bath/water bath (1.5mL centrifuge tube,95℃)；
9. Real-time PCR instrument with FAM/VIC/ROX/CY5 fluorescence channels(ABI7500,Bio-rad CFX96,QuantStudio,SLAN-96S,BTK-96).
The Wound Pathogen Panel is qualitative in vitro detection of 19 common pathogens DNA and 12 common drug(antibiotics) resistance(DR) genes from wound swab.
The pathogens and drug resistance genes detected are shown in the following [table 1].
[Table 1]
| Pathogen & DR gene type | Species |
Bacteria | Escherichia coli |
| Klebsiella aerogenes | |
| Klebsiella oxytoca | |
| Klebsiella pneumoniae | |
| Citrobacter spp.1 | |
| Enterobacter cloacae | |
| Acinetobacter baumannii | |
| Proteus mirabilis | |
| Pseudomonas aeruginosa | |
| Serratia marcescens | |
| Staphylococcus aureus | |
| Staphylococcus saprophyticus | |
| Streptococcus agalactiae | |
| Enterococcus faecalis | |
| Enterococcus faecium | |
| Morganella morganii | |
| Bacteroides fragilis | |
| Staphylococcus epidermidis | |
| Fungi | Candida albicans |
Drug resistance gene | blaKPC |
| blaNDM | |
| blaVIM | |
| blaIMP | |
| blaOXA-48 | |
| mecA | |
| vanA | |
| vanB | |
| CTX-M2 | |
| suL1 | |
| suL2 | |
| suL3 |
1. Include：Citrobacter freundii, Citrobacter werkmanii, Citrobacter portucalensis, Citrobacter youngae, Citrobacter cronae, Citrobacter braakii, Citrobacter arsenatis, Citrobacter amalonaticus, Citrobacter koseri.
2. Include：CTX-M 1 group, CTX-M 2 group, CTX-M 9 group.
The kit is designed with specific primers and probes for different common pathogens and drug resistance genes. Polymerase chain reaction (qPCR) and multiple fluorescent probe technique are used to amplify and detect specific nucleic acid sequences of pathogens and drug resistance genes listed in Table 1.
By adding edible yeast as internal control (IC), the extraction and detection process of pathogen nucleic acid from wound swab is monitored to effectively avoid false negative results. In order to avoid aerosol contamination of the amplified products, the UDG enzyme /dUTP system was added to the amplification system to effectively degrade the amplified products and avoid false positive results.
This kit is a fully premix freeze-dried system. Taq enzyme, UDG enzyme, reaction buffer, specific primers and probes required for amplification are all lyophilized in PCR tubes. A total of 1-8 Wells are lyophilized powders for different target genes. Detection can be performed directly after adding dissolving solution and extracted nucleic acid.
Model: PR2028-WP01
Format: Test cassette
Specimen: Wound swab
Storage: -25~-15℃(-13~5℉)
Shelf life: 12 months
| Components | 8 samples/kit | 24 samples/kit | Ingredient |
Wound Lyophilized Reagent | 8×8 strip tubes | 24×8 strip tubes | Specific primer&probes for the detection of pathogens and DR genes in Table 1, dNTP/dUTP Mix, Mg2+, Taq polymerase and UDG enzyme |
Internal Control Dry Powder | 1 tube | 1 tube | Edible yeast powder |
Internal Control Solution | 1 mL×1 tube | 1 mL×1 tube | Dnase/Rnase-free H₂O |
| Wound Positive Control | 300 μL×1 tube | 300 μL×1 tube | Plasmid containing every target gene sequence |
| Wound Negative Control | 300 μL×1 tube | 300 μL×1 tube | Plasmid containing internal control sequence |
| Dissolving Solution | 1 mL×1 tube | 1 mL×3 tubes | Stabilizer |
NOTE:
1. Do not mix the components from different batches for detection.
2. The kit can be transported at room temperature (no more than one month).
3. Repeated freezing and thawing should not exceed 7 times.
2). Precision: (CV, %) of Ct ≤5%.
3). Accuracy: The conformity rate of negative/positive reference 100%.
4). Specificity: Please refer to the User Manual.
1. Disposable powder free gloves and other personal protective equipment；
2. Pipettes (adjustable) and Sterile pipette tips；
3. Wound swab collection device；
4. 1.5 mL centrifuge tubes and racks；
5. Bench top centrifuge for centrifuge tubes and PCR tubes；
6. Bacterial nucleic acid extraction kit(It is recommended to use Nucleic Acid Extration Reagent manufactured by Bioteke, Item No.: BUR01)；
7. Nucleic acid extractor；
8. Metal bath/water bath (1.5mL centrifuge tube,95℃)；
9. Real-time PCR instrument with FAM/VIC/ROX/CY5 fluorescence channels(ABI7500,Bio-rad CFX96,QuantStudio,SLAN-96S,BTK-96).
