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DP1901/DP1902/DP1903
Bioteke
A. Principle
The Kit applies the unique binding buffer/ Proteinase K to rapidly lyse cells and inactivate cellualar nuclease, then DNA selectively absorbed on silica membrane in high salt solution, after that take a serial of elution- centrifugation step to remove cellular metabolite and proteins etc.
Finally use low salt elution to elute purified genome DNA from silica membrane.
B. Kit Components(DP1901)
| No. | Components | Specification | Qty |
| 1 | Buffer TB | 22ml | 1 |
| 2 | Buffer TL | 11ml | 1 |
| 3 | Buffer CB | 11ml | 1 |
| 4 | Buffer IR | 27ml | 1 |
| 5 | Buffer WB | 15ml(Add ration ethanol before use.) | 1 |
| 6 | Buffer EB | 15ml | 1 |
| 7 | Isopropano | 7ml | 1 |
| 8 | Protease K（20mg/ml） （For type II） | 20mg(dry powder) | 1 |
| 9 | Spin-column AC | 50pcs | 1 |
| 10 | Collection Tube（2ml） | 50pcs | 1 |
C. Storage and Validity
1. Stored at RT, Protease K should be stored at -20℃.
2. This kit shall be valid for 12 months. Please use it within its validity.
D. Features
1． The silica membrane in the spin-column comes from the world-famous company.
2． Poisonous phenol etc not used.
3． Procedure is simple and fast, single sample can be completed in 30 min.
4. Multi-elution can ensure high-purified DNA, the typical ratio of OD260/OD280 is 1.7～1.9, and the average length up to 30Kb-50kb, which can be applied for PCR，Southern-blot and digestions directly.
A. Principle
The Kit applies the unique binding buffer/ Proteinase K to rapidly lyse cells and inactivate cellualar nuclease, then DNA selectively absorbed on silica membrane in high salt solution, after that take a serial of elution- centrifugation step to remove cellular metabolite and proteins etc.
Finally use low salt elution to elute purified genome DNA from silica membrane.
B. Kit Components(DP1901)
| No. | Components | Specification | Qty |
| 1 | Buffer TB | 22ml | 1 |
| 2 | Buffer TL | 11ml | 1 |
| 3 | Buffer CB | 11ml | 1 |
| 4 | Buffer IR | 27ml | 1 |
| 5 | Buffer WB | 15ml(Add ration ethanol before use.) | 1 |
| 6 | Buffer EB | 15ml | 1 |
| 7 | Isopropano | 7ml | 1 |
| 8 | Protease K（20mg/ml） （For type II） | 20mg(dry powder) | 1 |
| 9 | Spin-column AC | 50pcs | 1 |
| 10 | Collection Tube（2ml） | 50pcs | 1 |
C. Storage and Validity
1. Stored at RT, Protease K should be stored at -20℃.
2. This kit shall be valid for 12 months. Please use it within its validity.
D. Features
1． The silica membrane in the spin-column comes from the world-famous company.
2． Poisonous phenol etc not used.
3． Procedure is simple and fast, single sample can be completed in 30 min.
4. Multi-elution can ensure high-purified DNA, the typical ratio of OD260/OD280 is 1.7～1.9, and the average length up to 30Kb-50kb, which can be applied for PCR，Southern-blot and digestions directly.
