For Research Use Only
The Multiple Respiratory Pathogens Nucleic Acid Detection Kit is qualitative in vitro test of 29 common respiratory pathogens in upper and lower respiratory tract samples.
The pathogens detected are shown in the following table 1:
[Table 1]
Virus | Covid 2019 | Bacteria | Klebsiella pneumoniae | Fungus | Aspergillus |
| Flu A, Flu B | Mycoplasma pneumoniae | Cryptococcus | |||
| Syncytial virus | Chlamydia pneumoniae | Pneumospora | |||
| Adenovirus | Streptococcus pneumoniae | ||||
| Enterovirus | Legionella pneumophila | ||||
Human metapneumovirus | Bordetella pertussis | ||||
| Human boca virus | Haemophilus influenzae | ||||
| Rhinovirus | Chlamydia psittaci | ||||
| Parainfluenza virus type 1, 2, 3, 4 | Group A streptococcus | ||||
| Coronavirus 229E | |||||
| Coronavirus OC43 | |||||
| Coronavirus NL63 | |||||
Coronavirus HKU1 |
Specific primers and probes were designed for common respiratory pathogens. Reverse transcription-polymerase chain reaction (RT-qPCR) and fluorescent probe technology were used to achieve the amplification and detection of specific nucleic acid sequences of pathogens in [Table 1], and RNaseP gene /UDG enzyme /dUTP system was added to avoid false negative and false positive results.
This kit is a fully premix freeze-dried system. Taq enzyme, UDG enzyme,reverse transcriptase, reaction buffer, specific primers and probes required for amplification are all lyophilized in PCR tubes. A total of 1-8 Wells are lyophilized powders for different target genes. Detection can be performed directly after adding dissolving solution and extracted nucleic acid.
| Components | 8 samples/kit | 24 samples/kit | Ingredient |
| RP06 Lyophilized Reagent | 8×8 strip tubes | 24×8 strip tubes | Specific primer&probes for the detection of pathogens and DR genes in [Table 1], dNTP/dUTP Mix, Mg2+, Taq polymerase, reverse transcriptase, and UDG |
| RP06 Positive Control | 200 μL×1 tube | 400 μL×1 tube | Plasmid containing every target gene sequence |
| Negative quality control | 200 μL×1 tube | 400 μL×1 tube | Plasmid containing human RNaseP gene sequence |
| Rehydration solution | 1 mL×1 tube | 1 mL×3 tubes | Stabilizer |
Note:
Components of different batches cannot be mixed.
Sample nucleic acid extraction should be carried out at the same time with negative quality control products and positive quality control products.
Freeze-dried reagents are sealed packaging, please use immediately after opening, do not expose to the air for a long time. Freeze-dried reagent is encapsulated in 0.2mL PCR eight-tube, if the fluorescent PCR instrument used cannot use the consumable, the liquid of each hole should be imported and applied before loading the machine consumables.
The kit can be transported at room temperature(no more than 1 month).
Repeated freezing and thawing not exceed 7 times.
Model: PR2022-RP06
Format: Test cassette
Specimen: Oropharyngeal swab, Nasopharyngeal swab, Sputum, Alveolar lavage fluid
Storage: -25~-15℃(-13~5℉)
Shelf life: 12 months
1). Multiplex Testing
Customized selections can be made based on the pathogens that customers need to detect, and test results for a variety of pathogens can be obtained.
2). Stable and Reliable
This kit is a freeze-dried PCR system. The active substances required in the detection process (enzymes, dNTPs, primer probes, etc.) are pre-placed in the system. It supports room temperature transportation within 1 month and has good storage stability, ensuring reliable and accurate test results.
3). Fast and Convenient
The whole process of testing with this reagent can be completed within 90 minutes. If used with PCR equipment, the testing time can be shortened to 50 minutes. In addition, this system does not need liquid preparation and packaging operations, saving time and labor costs.
4). Sensitive and Accurate
The analytical sensitivity of this kit is 1000 copies/mL. It includes human internal standard monitoring, the entire process from sampling, nucleic acid extraction to nucleic acid detection, to avoid false negatives. The detection system always includes the UDG/dUTP system, which can effectively avoid false negative results caused by contamination of amplification products.
Performance Index
1. The analytical sensitivity of this kit was 1000 copies/mL.
2. Precision: The coefficient of variation (CV, %) of precision Ct value within/between batches is less than 5%.
3, accuracy: the test enterprise negative/positive reference conformity rate is 100%.
1. Disposable powder free gloves and other personal protective equipment；
2. Disposable sampling consumables and storage tubes;
3. Pipettes (adjustable) and Sterile pipette tips；
4. Nucleic acid extraction/purification reagent and matching nucleic acid extractor；
5. 1.5 mL RNase-free centrifuge tubes and racks；
6. Bench top centrifuge for centrifuge tubes and PCR tubes；
7. Real-time PCR instrument with FAM/VIC/ROX/CY5 fluorescence channels(ABI7500,Bio-rad CFX96,QuantStudio,SLAN-96S,BTK-96).