Urinary Tract Infection And Drug Resistance Detection Tool: Bioteke's Fast And Accurate Solution

Urinary tract infection (UTI) & Drug Resistance Gene Detection Kit

-- Fast and accurate diagnostic solution

Background

• Urinary tract infection (UTI) is a common and highly prevalent disease worldwide, and its incidence rate ranks second only to respiratory tract diseases among infectious diseases.

• According to foreign statistics, the incidence rate of UTI is 2-5%, which is an infectious disease with high incidence and mortality, and seriously threatens human health and life safety.

• According to relevant statistics from the World Health Organization (WHO), 130 million to 175 million people suffer from UTI every year worldwide, and UTI has become the second largest infectious disease after respiratory tract infection. It is estimated that in the United States, 13.3% (12.8 million) of women and 2.3% (2 million) of men are infected with UTI every year, resulting in the U.S. medical system spending about $3.5 billion each year.

• However, in the past 10 years, with the extensive application of broad-spectrum antimicrobial drugs in clinical and animal husbandry fields, the resistance spectrum of pathogens has shifted, and the problem of drug resistance has become increasingly serious. The empirical use of antibiotics in clinical treatment may delay the best treatment time and lead to the abuse of antibiotics. Therefore, the diagnosis of pathogens and the prediction of drug resistance are very important in guiding the drug treatment of patients with urinary tract infections.

• Urine is sterile under normal circumstances, but it does not mean that all bacteria in urine are pathogenic bacteria. Only when the bacteria detected in urine can grow and multiply in the urinary tract and cause infection can they be diagnosed as pathogenic bacteria of urinary tract infection. Urinary tract pathogens are invasive and can invade and colonize the urothelium and multiply in urine. More than 95% of pathogens of urinary tract infection are single species of bacteria. There are also a small number of complicated urinary tract infections caused by multiple pathogens.

• At present, the gold standard for diagnosing pathogens of urinary tract infection is urine bacterial isolation, culture, identification and drug sensitivity analysis. However, the culture method has a long cycle, usually 5-7 days, and has a low positive rate and limited application range (some pathogens are difficult to culture in vitro). Due to the long time from onset to report, it is easy to miss the best treatment period in the acute phase.In recent years, nucleic acid testing has been increasingly used in the diagnosis of urinary tract infection pathogens worldwide. The target of nucleic acid testing is the genetic material of the pathogen. By selectively amplifying the target sequence, very high detection sensitivity and specificity can be achieved. Since nucleic acid testing does not require bacterial isolation and culture, it can quickly and sensitively detect pathogens in the early stages of the disease.

  
  

Bioteke UTI & DR Detection Project

• This project intends to develop a nucleic acid detection kit for urinary tract infection pathogens and drug-resistant genes based on an innovative nucleic acid rapid detection platform and patented fluorescent PCR freeze-drying technology to guide clinical treatment in the early stages of the disease.

• Bioteke Urinary Tract Infection (UTI) & DR Panel (Fluorescence PCR Method) is used for in vitro qualitative detection of 19 common pathogen nucleic acids and 12 common drug-resistant genes in urine samples.

• Bioteke Urinary Tract Infection (UTI) & DR Panel (Fluorescence PCR Method) designs specific primers and probes for different pathogens and drug-resistant genes, and uses polymerase chain reaction (qPCR) and multiple fluorescent probe technology to amplify and detect specific nucleic acid sequences of pathogens and drug-resistant genes. By adding edible yeast as an internal standard (IC), the nucleic acid extraction and detection process of pathogens in urine is monitored to effectively avoid false negative results. In order to avoid aerosol contamination of the amplification product, the UDG enzyme/dUTP system is added to the amplification system to effectively decompose the amplification product and avoid false positive results.

• This kit is a fully premixed freeze-dried system. The Taq enzyme, UDG enzyme, reaction buffer, specific primers and probes required for amplification are all freeze-dried in PCR tubes. Wells 1-8 contain freeze-dried powders for different target genes. Each well uses 4-color fluorescence channels for detection, and a total of 31 target analyte nucleic acids are detected. The method of use is simple and can be directly tested after adding the reconstitution solution and the extracted sample nucleic acid.

Significance of the Project

• Infectious diseases have always been an important public health issue worldwide. In its diagnosis and treatment, rapid and accurate identification of pathogens is crucial. Traditional detection methods mainly include morphological detection, culture separation, biochemical detection, immunology and nucleic acid detection, which are simple to operate, relatively low in detection cost, and have good sensitivity and specificity. They are still widely used in clinical practice. Although metagenomic mNGS nucleic acid detection technology can overcome the problem of single pathogen detection, it has high requirements for equipment, personnel, and costs.

• The use of multi-target multiplex fluorescence quantitative PCR technology in this field basically overcomes the shortcomings of other detection methods, such as long time, complex operation, and high personnel requirements.

1. Guide medication: Clinically, medication is mostly based on experience in the treatment of urinary tract infections, especially in the blind use of antibiotics. Medication based on pathogen identification is the key to improving patient prognosis.

2. Find the cause of the disease in a timely manner: Although the traditional bacterial culture method has high accuracy, it is time-consuming, has a considerable proportion of false negatives, and is prone to delay treatment. Nucleic acid multiplex fluorescence quantitative PCR detection can identify pathogens in an early stage and use symptomatic medication in time, so as to better improve the prognosis of patients with urinary tract infection.

3. Improve the hospital's detection capabilities: After this method is established in the hospital, it can not only provide better detection methods for patients coming to the hospital, but also provide a new detection method for the hospital. At the same time, it also provides more comprehensive medical basic data for the clinic.