Publish Time: 2025-02-14 Origin: Bioteke
During the influenza pandemic season, medical institutions and laboratories face the challenge of a surge in co-infections with respiratory pathogens.
The early symptoms of pathogens such as the new coronavirus, influenza virus (type A and type B), respiratory syncytial virus, adenovirus, and Mycoplasma pneumoniae are highly similar. If they cannot be quickly distinguished, it is easy to lead to misdiagnosis or delayed treatment, especially posing a threat to high-risk groups such as the elderly and infants.①③
Therefore, efficient, rapid, and accurate multi-pathogen simultaneous detection technology has become the key to diagnosis and treatment efficiency.
Synergistic effect of antigen and PCR technology
1. Rapid antigen test: Initial screening and emergency management
Antigen testing is known for its rapidity (results in 15-20 minutes) and convenience (no laboratory equipment required), and is suitable for large-scale screening and community monitoring. However, its limitations should be noted:
When the viral load is low, the sensitivity is low.⑧⑩
Positive results require PCR verification to exclude false positives and confirm the type of infection.⑧
Bioteke 8 in 1 test
Bioteke 10 in 1 test
2. PCR technology: the “gold standard” for diagnosis and simultaneous detection of multiple pathogens
Nucleic acid testing (such as fluorescent quantitative PCR) has become a core tool for diagnosing and identifying mixed infections due to its high sensitivity (can detect viral RNA as low as 20 copies/reaction) and specificity (100%).④⑨
Respiratory multiple pathogen nucleic acid detection kit (fluorescence PCR method) developed by Bioteke meets clinical needs through the following advantages:
Multi-target coverage
A single test can simultaneously screen 29 pathogens such as COVID-19, influenza A and B, syncytial virus, and adenovirus, significantly improving diagnostic efficiency①③.
Technical optimization
Premixed freeze-drying technology is used to monitor the effectiveness of the entire process of sample sampling, nucleic acid extraction and nucleic acid detection by detecting the human RNaseP gene as an internal standard to avoid false negative results.
To avoid aerosol contamination of amplification products, the UDG enzyme/dUTP system is added to the amplification system to effectively decompose the amplification products and avoid false positive results③⑨.
Stable transportation and storage
The freeze-dried system tolerates temperature fluctuations and is suitable for global transportation and use in resource-poor areas.
Respiratory PCR kit
| Dimensions | Bioteke multipathogen PCR kit | Traditional single pathogen test |
| Detection efficiency | Covers 29 pathogens in one shot | Multiple tests, time-consuming and material-consuming |
| Sensitivity | High, 1000copies/mL | Depends on pathogen specificity and reagent sensitivity |
| Convenience of operation | Premixed lyophilized type, no reagent preparation required | Requires multiple pipetting and centrifugation steps |
| Cost-effectiveness | Reduce repeated testing and low overall cost | High cost per test |
1). Layered Detection
Initial screening:
Rapid antigen testing is used for patients with mild symptoms or asymptomatic patients.
Confirmation:
PCR kits are used for positive or suspected cases to ensure accurate typing③⑩.
2). Resource Integration
Combine automated equipment (such as fully automatic nucleic acid extraction instruments) with manual review to balance speed and accuracy⑦.
Establish a data sharing platform to report positive results to the public health system in real time to help monitor the epidemic⑥⑨.
3). Staff training and quality control
Regularly calibrate PCR equipment to ensure that the storage conditions of the test kit meet the requirements⑨.
Train laboratory personnel to master cross-contamination prevention and control measures for multiple tests (such as nucleic acid aerosol removal devices)⑦.
Towards a future of precision medicine
In the "post-epidemic era" where influenza and COVID-19 coexist, the technical solution of integrating antigen screening and multiplex PCR diagnosis can significantly improve the diagnostic efficiency and patient management capabilities of medical institutions. Bioteke Corporation provides reliable solutions for global medical institutions with its high sensitivity, multi-pathogen coverage and convenient operation.
By optimizing the testing process and resource allocation, we hope to achieve faster and more accurate responses in future public health crises.
For further details or purchase the kits, please contact Bioteke team.
Website: www.bioteke.cn
Phone/Whatsapp: +86 18115363743
E-mail: zr@bioteke.cn
Address: No.9 Huiming Rd, Huishan District, Wuxi City, 214174, China.
[Reference]
Bioteke Corporation ①③⑨
Thermo Fisher Scientific ⑥
BMC Microbiology Research ④
BBC&ABC news ⑧⑩
Telephone/Whatsapp: +86 13357927939