Views: 0 Author: Site Editor Publish Time: 2021-10-29 Origin: Site
Sampling kits are used for the collection, preservation, and transportation of virus specimens such as novel coronaviruses, influenza viruses, avian influenza (e.g. H7N9), hand, foot, and mouth viruses, measles, as well as mycoplasma, urea plasma, and chlamydia specimens. So why do we need to choose a special sampling kit?
Here is the content list:
l The need to choose a quality sampling kit
l What is a good virus sampling tube like?
l Considerations when selecting sampling kits
The quality of the sample collected by the sampling kit is directly related to the subsequent detection. If improper collection tools and preservation liquids are used, the collected samples have a low viral load and gradually decompose with changes in the survival microenvironment, which can easily cause wrong detection results and make it difficult to confirm the diagnosis. Therefore, the use of special virus sampling kits can guarantee the validity of the sample and reduce false negatives!
A superior virus sampling kit on the one hand can improve the abundance of viruses when collecting samples, and on the other hand, can sampling kit can guarantee the activity and integrity of virus particles to the maximum extent, providing the first guarantee for subsequent virus isolation and nucleic acid detection.
(1) It is important to maintain the activity of the pathogen infection factor;
(2) Sampling kits can meet the sample collection to laboratory testing time;
(3) The need to limit or prevent the proliferation of commensal microorganisms to ensure the reliability of diagnostic tests;
(4) The integrity of the viral antigens and nucleic acids must be maintained;
(5) Sampling kits are capable of maintaining viral activity at ambient conditions.
Virus sampling kits are loaded with infectious substances, some even highly pathogenic infectious substances, therefore, the requirements of the sampling kit container are very demanding, to meet the requirements of safety at the same time
To ensure that the sample does not leak during the transportation process.
To ensure that the sample does not leak during the preservation process.
Virus preservation fluid.
1. There are two main types widely used in sampling kits, one is a modified virus maintenance fluid type preservation solution based on transport medium, and the other is a modified lysis type preservation solution for nucleic acid extraction lysate. Because the samples collected in the sampling kit may subsequently involve a variety of testing methods, not only for nucleic acid extraction of viruses but also for virus culture, isolation, and antigen detection, etc.
2. It is recommended to choose the virus maintenance fluid type preservation solution, which can maintain the originality of the sample to the greatest extent possible so that it can be used for a variety of testing methods. However, it should be noted that no matter which preservation fluid type sampling kit is selected, strict inactivation should be performed before testing.
Sampling kits for encapsulated preservatives.
Sampling kit material is appropriate to use polyethylene - propylene polymer (Polyallomer) plastic and some special treatment of polypropylene (PP), polycarbonate (PC) plastic and other containers, need to be airtight, high and low-temperature resistance, can be suitable for a variety of inactivation methods.
Sampling swab:
1. Sampling kit swab head should be used synthetic fiber flocking is appropriate, should not choose cotton or calcium alginate sponge and other materials;
2. The material of the swab handle is recommended to use hollow polystyrene (PS) extruded tube or polypropylene (PP) injection indentation tube, not wooden sticks or bamboo sticks, and other materials. Because the wood stick or bamboo swab containing calcium alginate and wooden components soaked in preservation solution after breaking will adsorb proteins, and will even interfere with the subsequent sample test in the sampling kit, the fiber of cotton has stronger adsorption of proteins and is not easy to elute.
3. Swab length should not be too short, and the fracture position should not be too close to the head of the swab, so as not to stain the patient's body fluids during operation;
4. The fracture point should be reasonably located to avoid the risk of contamination caused by the spill of liquid inside the sampling kit when the bag is fractured.
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