microRNA Fast Extraction Kit (Spin-column)
A. Principle
The kit applies improved guanidine thiocyanate-phenol one-step method to lyse samples and inactivate ribonucleases, then genomic DNA and 18 and 28s RNA are removed by the first spin-column. Then microRNA (including miRNA, snRNA,and other RNA less than 200bp) is absorbed by the second spin-column.
After a serial of elution-centrifugation steps to remove cellular metabolite and proteins etc, finally microRNA will be eluted from silica membrane using low salt RNase-free water.
B. Kit Components(RP5301)
| No. | Components | Specification | Qty |
| 1 | Buffer MRL | 55ml | 1 |
| 2 | Buffer RW | 15ml | 1 |
| 3 | RNase-free H2O | 10ml | 1 |
| 4 | Buffer RW | 15ml | 1 |
| 5 | RNase-free Spin-column RA | 50pcs | 1 |
| 6 | RNase-free Spin-column RB | 50PCS | |
| 7 | 70% ethanol | 9ml RNase-free H2O | 1 |
| 8 | Collection Tube（2ml） | 50pcs | 1 |
C. Storage and Validity
1. Stored at RT(15-25℃), Buffer MRL should be stored at 4℃ in the dark.
2. This kit shall be valid for 12 months. Please use it within its validity.
D. Features
1． The silica membranes in the spin-column are produced by the world-famous company. There is little variation among different spin-columns, so repeatability of tests is reliable!
2． MicroRNA can be eluted from silica membrane without ethanol precipitation and dissolve.
3． The buffer MRL contains some special components can clean up the contamination of genomic DNA efficiently.
4． Multi-elution can ensure high-purity microRNA.
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