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A. Principle
This kit can be used to isolate RNA from whole blood, biological fluids, and other liquid samples.
The RNzol LS procedure represents a well-established technology for RNA purification. This technology combines the selective binding properties of a silica-based membrane with the speed of microspin technology.
A specialized high-salt buffer system allows up to 100 μg of RNA longer than 200bp binding to the RNzol LS silica membrane. Biological samples are first lysed and homogenized in the presence of a highly denaturing guanidine-thiocyanate–containing buffer, which immediately inactivates RNases to ensure purification of intact RNA.
Ethanol is added to provide appropriate binding conditions, and the lysate is transferred to the spin-column, where the total RNA binds to the membrane and contaminants are efficiently removed. High-quality RNA is then eluted in 30-100 μl water.
B. Kit Components(RP4001)
| No. | Components | Specification | Qty |
| 1 | Buffer RLS | 55ml | 1 |
| 2 | Buffer RE | 30ml | 1 |
| 3 | RNase-free H2O | 10ml | 1 |
| 4 | Buffer RW | 15ml(Add ration ethanol before use.) | 1 |
| 5 | RNase-free Spin-column AC | 50pcs | 1 |
| 6 | 70% ethanol | 9ml RNase-free H2O | 1 |
| 7 | Collection Tube（2ml） | 50pcs | 1 |
C. Storage and Validity
1. Stored at RT(15-25℃), Buffer RLS should be stored at 4℃.
2. This kit shall be valid for 12 months. Please use it within its validity.
D. Features
◆ Convenience, no need to lyse erythrocytes and separate leukocytes for isolation total RNA from whole blood.
◆ Stability, comparable RNA yield with high quality adsorbing membrane.
◆ High-purity, specific membrane absorption and wash-elution system ensure to remove protein and other debris.
Telephone: +86-510-8332 3992