Effective Urinary Tract Infection(UTI) Diagnosis: PCR Kits Tailored for Healthcare Professionals

Effective Urinary Tract Infection(UTI) Diagnosis

PCR Kits Tailored for Healthcare Professionals

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Background

Urinary tract infection(UTI) is an infectious disease with a high incidence rate second only to respiratory diseases worldwide. 

According to statistics, 130-175 million people suffer from urinary tract infection every year, and its incidence rate is 2%-5%. In the USA, about 13.3% of women and 2.3% of men suffer from infection every year, resulting in an annual medical system cost of about $3.5 billion.

The main treatment for urinary tract infection is antibiotics. However, the abuse of antibiotics has led to the rapid spread of drug-resistant strains, posing a huge challenge to clinical treatment. Therefore, accurate diagnosis and drug-resistant gene detection for urinary tract infection have become the focus of academic research and public health.

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Infectious Factors

Urinary tract infection is often caused by invasive procedures.

Invasive procedures can bring bacteria into the urethra and bladder, causing urinary tract damage and infection.

Mainly including invasive procedures of the clinical urinary system such as cystoscopy, retrograde pyelography, and catheterization.

Among patients with indwelling catheters, 2%-4% will develop bacteremia and sepsis, with a mortality rate of 13-30%.

According to the US Centers for Disease Control(CDC) and Prevention, urinary tract infections caused by indwelling catheters account for 42%; a British study reported that urinary tract infections caused by indwelling catheters accounted for 30%; a survey of 2,772 hospitalized patients by Hiroshima University in Japan showed that 33% of them had urinary tract infections, of which 93% were caused by indwelling catheters.

Normally, urine is sterile, but this does not mean that the bacteria in urine are pathogenic.

Only when the bacteria detected in urine can grow and multiply in the urinary tract and cause urinary tract infection, can it be diagnosed as pathogenic bacteria.

Urinary tract pathogens are invasive and can invade and colonize the urothelium and multiply in urine. More than 95% of the pathogens of urinary tract infection are single species of bacteria; however, there are also a small number of urinary tract infections caused by multiple pathogens.

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Diagnostic Tests for Urinary Tract Infections

At present, the "gold standard" for the diagnosis of urinary tract infection pathogens is urine bacterial isolation, culture, identification, and drug sensitivity analysis.

In recent years, nucleic acid detection methods have been increasingly used in the diagnosis of urinary tract infection pathogens.

Urinary tract infection nucleic acid detection pathogens are divided into sequencing and amplification methods in principle. The amplification method based on PCR technology detects urinary tract infection and drug resistance genes, which is more popular, has cost advantages, and takes less time.

METHOD	Urine Culture	mNGS Sequencing	Fluorescence Quantitative PCR
Throughput	Low	High, More than 100 kinds	More than 30 kinds, and scalable
Detection Items	Pathogens and drug resistance sensitivity	Pathogens and drug resistance genes	Pathogens and drug resistance genes
Operability	Higher difficulty	Complex	Relatively simple
Cost	Expensive	Expensive	Cheaper
Time	Routine culture 2-3 days, drug sensitivity test ≥ 5 days	≥2 Days	≤ 1 Day
Requirements	Aseptic operation, some pathogens are difficult to culture	High equipment cost and high data analysis requirements	Easier result determination
Equipment	Incubator, etc.	Nucleic Acid Sequencer	Fluorescence quantitative PCR instrument
Sensitivity	Prone to false negative results	High sensitivity	High sensitivity

_{Note: The comparison results show that the fluorescence quantitative PCR method has greater advantages.}

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Bioteke Corporation UTI & DR Detection Solutions

Based on the innovative rapid nucleic acid detection platform and patented fluorescent PCR freeze-drying technology, Bioteke Urinary Tract Infection(UTI) & DR Panel(Fluorescence PCR Method), which helps in early diagnosis of the disease and clinical treatment guidance.

• Intended Use

In vitro qualitative detection of 19 common pathogen nucleic acids and 12 drug resistance genes in urine samples.

• Test Principle

1). Specific primers and probes are designed for different pathogens and drug-resistant genes, and polymerase chain reaction (qPCR) and multiple fluorescent probe technology are used to amplify and detect specific nucleic acid sequences of pathogens and drug-resistant genes.

2). Add internal standard (IC) to avoid false negative results; add UDG enzyme/dUTP system to avoid false positive results.

3). Use a fully pre-mixed freeze-dried system to freeze-dry the Taq enzyme, UDG enzyme, reaction buffer, specific primers and probes required for the amplification experiment in PCR tubes, which is more convenient to use.

• Application Scenario

1. Clinical guidance

Improve the clinical empirical medication in the treatment of urinary tract infections, especially the use of antibiotics.

2. Accurate diagnosis

The fluorescent PCR detection method takes less time than the urine culture method, and can detect pathogens earlier and more accurately, helping to provide timely symptomatic treatment.

3. Capacity improvement

This product can be used as a new diagnostic test method for medical laboratories, hospitals and other institutions, providing better detection methods. Provide more comprehensive medical basic data reference for clinical practice.

Product Features and Advantages

High analytical sensitivity

200 CFU/mL

Rapid detection

Freeze-dried reagent, add sample, cooperate with fast PCR instrument, and complete the detection in 60 minutes.

Multiple targets

Can detect 19 common pathogens and 12 drug-resistant genes at the same time.

Freeze-dried system

Simplify the operation process, reduce transportation risks, and have higher stability.

Widely used

Can be used for routine urology testing, prognosis monitoring, third-party laboratories, CDC, scientific research institutions, etc.

Summary

The importance of urinary tract infection and drug resistance gene detection in the medical and scientific research fields cannot be ignored. 

Bioteke's urinary tract infection drug resistance gene detection kit provides efficient and accurate solutions for medical workers and researchers through innovative fluorescent PCR freeze-drying technology. 

If you are looking for a high-performance PCR kit, Bioteke is your ideal choice.

For more details, please visit our official website or contact our technical support team directly.

Web: www.bioteke.cn

E-mail: zr@bioteke.cn

Tel/Whatsapp: +86 18115363743

Address: No.9 Huiming Road, Huishan District, Wuxi, Jiangsu Province, China 214174.

Reference：

1．Warrell D, M. Cox T, Firth J, Torok E. A checklist of bacteria associated with infection in humans. Oxford Textbook of Medicine: Infection. (2012) 6.46. 

2．RB Jesús,Lorena L C,MD Navarro,et al. Faecal carriage of extended-spectrum beta-lactamase-producing Escherichia coli: prevalence, risk factors and molecular epidemiology.[J].8 / 8 Journal of Antimicrobial Chemotherapy, 2008(5):1142-9. 

3．Yao, Wang, Xiuli, et al. In vitro susceptibilities of aerobic and facultative gram-negative bacilli isolated from patients with intra-abdominal infections and urinary tract infections in China:the 2010 Study for Monitoring Antimicrobial Resistance Trends (SMART)[C].2012 Beijing Laboratory Medicine Annual Meeting and Laboratory Medicine Forum for Young and middle-aged people. 

4．Lipsky B A , Byren I , Hoey C T . Treatment of bacterial prostatitis.[J]. American Family Physician, 2010, 50(12):1641-1652. 

5．Schwartz D J , Chen S L , Hultgren S J , et al. Population Dynamics and Niche Distribution of Uropathogenic Escherichia coli during Acute and Chronic Urinary Tract Infection[J]. Infection and Immunity, 2011, 79(10):4250-4259.