A. Principle
Unique extracting and lysis system, not only quickly lyses cells, but also inactivates intracellular nuclease. Furthermore, it easily keeps the integrity of genomic DNA, Purification column after special treatment can effectively remove the impurity and humic acid. It is also fast, quick. DNA is suitable for PCR and other downstream reactions.
B. Kit Components(DP4611)
| No. | Components | Specification | Qty |
| 1 | Extraction Buffer | 50ml | 1 |
| 2 | Buffer A | 750ul | 1 |
| 3 | Buffer B | 5ml | 1 |
| 4 | Buffer C | 25ml | 1 |
| 5 | Protein precipitation Buffer | 18ml | 1 |
| 6 | Elution Buffer EB | 5ml | 1 |
| 7 | Purification Column | 50pcs | 1 |
| 8 | Collection Tube | 50pcs | 1 |
C. Storage and Validity
1. Stored at RT. Buffer A should be stored at -20℃.
2. This kit shall be valid for 12 months. Please use it within its validity.
D. Features
Ø Purification column after special treatment can effectively remove the impurity and humic acid.
Ø High compatibility, it can be used for different kinds of stool, soil, feces.
Ø It does not contain some toxic solution such as phenol, and also no ethanol precipitation required.
Ø Fast, simple, the single sample can be finished in 60 min.
Ø High purity, the value of OD260/OD280 achieves 1.7-1.9,which can be applied to PCR，Southern-blot and digestions directly.
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