A. Principle
The kit applies the unique binding buffer/ Proteinase K to rapidly lyse cells and inactivate cellular nucleases, and then DNA selectively adsorbs on silica membrane in high salt solution.
Take a serial of elution-centrifugation steps to remove cellular metabolites and proteins etc, finally use low salt solution to elute the purified genomic DNA from silicified membrane.
B. Kit Components(DP2001)
| No. | Components | Specification | Qty |
| 1 | Buffer RB | 44ml | 1 |
| 2 | Buffer CB | 11ml | 1 |
| 3 | Buffer IR | 27ml | 1 |
| 4 | Buffer WB | 15ml(Add ration ethanol before use.) | 1 |
| 5 | Buffer EB | 15ml | 1 |
| 6 | Isopropano | 7ml | 1 |
| 7 | Protease K（20mg/ml） （For type II） | 20mg(freezed-dried powder) | 1 |
| 8 | Spin-column AC | 50pcs | 1 |
| 9 | Collection Tube（2ml） | 50pcs | 1 |
C. Storage and Validity
1. Stored at RT, Protease K should be stored at -20℃.
2. This kit shall be valid for 12 months. Please use it within its validity.
D. Features
1． The silica membranes in the spin-column come from the world-famous company.
2． Poisonous phenol etc is not used.
3． Provide a very simple and fast way; single sample can be completed in 30 min.
4． Multi-elution can ensure high-purified DNA. The typical ratio of OD260/OD280 is 1.7～1.9, and the average length is 30kb-50kb. Purified DNA can be applied for PCR，Southern-blot and digestions directly.
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