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A. Intended Use
For isolation and purification of high-quality viral DNA / RNA from cells, blood, serum, plasma, lymph, acellar fluid, cell culture supernatant or various preserving fluid.
B. Principle
The kit applies the unique binding buffer/ Protease K to rapidly lyse nuclease of virus and inactivate virus. Then genome DNA and RNA selectively adsorbs to silicified membrane in high salt solution. Impurities such as cellular metabolite and proteins etc. are removed through a series of of elution- centrifugation steps. Finally purified genome DNA and RNA is eluted from silica membrane by low salt elution buffer EB.
C. Kit Components(AU4611)
| No. | Components | Specification | Qty |
| 1 | 1*PBS | 10ml | 1 |
| 2 | Virus lysis buffer VL1 | 25ml | 1 |
| 3 | Proteinase K(40mg/ml) | 40mg | 1 |
| 4 | Buffer RW | 15ml | 1 |
| 5 | Eluting Buffer EB | 10ml | 1 |
| 6 | Spin Column VI | 50pcs | 1 |
| 7 | Collection Tube（2mL） | 50pcs | 1 |
| 8 | Lab water | 1ml | 1 |
D. Storage and Validity
1. Proteinase K (dry powder) is stored under -20±5℃; Buffer RW can be stored under 2~8℃ for one month, and -20±5℃ for one year； other components are stored under room temperature.
2. This kit shall be valid for one year. Please use it within its validity.